DNA

Part:BBa_K4747001:Design

Designed by: Liu Lantian   Group: iGEM23_SUSTech-MED   (2023-10-11)


araBAD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56


Design Notes

To construct vectors for aiiA/ytnP/wspF/yhjH expression


Source

The araBAD gene is part of the arabinose operon in bacteria like E. coli

References